Fluorescence Software

Phase Contrast/DIC

Colocalization

Original image by Flickr/mamarie. CC BY-SA 2.0.

Cells expressing fluorescently-labeled HSP70 (green) and/or P73α (red).

Fully automated count of nuclei, with the percentage expressing HSP70, P73α, or both.

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Sub-cellular Localization

Original image by 8x57is/Wikimedia Commons. CC BY-SA 4.0.

Mitochondrial distribution within a differentiated PC12 cell

Fully automatic detection of mitochondria, separation of neurites and cell body, and measurement of mitochondrial fraction in each.

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Cell Polarization

Polarized Myosin-GFP in a C. elegans zygote

Fully automated detection of anterior and posterior domains and measurement of GFP fluorescence

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Original image courtesy of L. E. Small

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